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Endale, A., Kammerer, B., Gebre-Mariam, T., & Schmidt, P. C. (2005). Quantitative determination of the group of flavonoids and saponins from the extracts of the seeds of glinus lotoides and tablet formulation thereof by high-performance liquid chromatography. Journal of Chromatography A, 1083(1-2), 32–41. |
| Resource type: Journal Article DOI: 10.1016/j.chroma.2005.05.095 ID no. (ISBN etc.): 219673 BibTeX citation key: Endale2005 View all bibliographic details  |
Categories: General Keywords: Glinus lotoides, Glinuside G, Mollugogenol B, Quantitative determination, RP-HPLC, Vicenin-2 Creators: Endale, Gebre-Mariam, Kammerer, Schmidt Collection: Journal of Chromatography A |
| Abstract |
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{The total flavonoids and saponins of the seeds of Glinus lotoides in the crude extracts and tablet formulation thereof were quantified by reversed-phase high-performance liquid chromatographic (RP-HPLC) methods with UV detection. The saponins were analyzed after acid hydrolysis in 3 M HCl at 100°C for 1 h. Vicenin-2 and mollugogenol B were isolated and used as reference substances for the quantification of total flavonoids and saponins, respectively. The identity and purity (>97%) of the standards were confirmed by spectroscopic (UV, MS, and NMR) and chromatographic (HPLC) methods. The flavonoids and saponins of the crude extract of the seeds and tablet formulation were separated by RP-HPLC (Nucleosil RP-18 column, 250 mm × 4.6 mm) using linear gradient elution systems of acetonitrile-water-0.1 M H3PO4 for flavonoids and methanol-water for saponins. Satisfactory separation of the compounds was obtained in less than 30 and 25 min, for the flavonoids and saponins, respectively. The methods were validated for linearity, repeatability, limits of detection (LOD) and limits of quantification (LOQ). Repeatability (inter- and intra-day
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